{"id":6338,"date":"2014-11-25T17:07:02","date_gmt":"2014-11-25T09:07:02","guid":{"rendered":"https:\/\/2blog.ilc.edu.tw\/6497\/2014\/11\/25\/103-%e8%a8%82%e5%ae%9a%e5%af%a6%e9%a9%97%e5%ae%a4%e5%ae%89%e5%85%a8%e5%ae%88%e5%89%87%e5%8f%8a%e7%ae%a1%e7%90%86%e8%be%a6%e6%b3%95\/"},"modified":"2021-03-18T18:37:02","modified_gmt":"2021-03-18T10:37:02","slug":"103-%e8%a8%82%e5%ae%9a%e5%af%a6%e9%a9%97%e5%ae%a4%e5%ae%89%e5%85%a8%e5%ae%88%e5%89%87%e5%8f%8a%e7%ae%a1%e7%90%86%e8%be%a6%e6%b3%95","status":"publish","type":"post","link":"https:\/\/2blog.ilc.edu.tw\/6497\/2014\/11\/25\/103-%e8%a8%82%e5%ae%9a%e5%af%a6%e9%a9%97%e5%ae%a4%e5%ae%89%e5%85%a8%e5%ae%88%e5%89%87%e5%8f%8a%e7%ae%a1%e7%90%86%e8%be%a6%e6%b3%95\/","title":{"rendered":"103 \u8a02\u5b9a\u5be6\u9a57\u5ba4\u5b89\u5168\u5b88\u5247\u53ca\u7ba1\u7406\u8fa6\u6cd5"},"content":{"rendered":"<p style=\"text-align: center\"><strong><span style=\"font-family:\u6a19\u6977\u9ad4;font-size:24pt\">\u5be6\u9a57\u5ba4\u5b89\u5168\u5b88\u5247<\/span><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\"><span style=\"font-size:18pt\"><br \/>\n\t\t\t\t<\/span><span style=\"font-size:24pt\"><br \/>\n\t\t\t\t<\/span><\/span><\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/2blog.ilc.edu.tw\/wp-content\/uploads\/sites\/2680\/6497\/6497-2816126.png\" alt=\"\" \/><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\"><br \/>\n\t\t<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 14pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\"><strong>\u4e00\u822c\u898f\u5b9a <\/strong><br \/>\n\t\t<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">1.\u4e0a\u8ab2\u7b2c\u4e00\u5929\u8acb\u5148\u719f\u6089\u74b0\u5883\uff0c\u5bdf\u770b\u7dca\u6025\u6c96\u6d17\u7ad9\u3001\u6d17\u773c\u7ad9\u3001\u6ec5\u706b\u5668\u3001\u6025\u6551\u7bb1\u53ca\u5b89\u5168\u68af\u7b49\u7684\u4f4d\u7f6e\uff0c\u7262\u8a18\u300c\u5b89\u5168\u300d\u662f\u9032\u884c\u4efb\u4f55\u5be6\u9a57\u6700\u91cd\u8981\u7684\u8003\u91cf\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">2.\u5728\u5be6\u9a57\u5ba4\u5167\u8acb\u7a7f\u8457\u5be6\u9a57\u8863\uff08\u6700\u597d\u9577\u53ca\u819d\u84cb\u4e0b\uff09\uff0c\u907f\u514d\u7a7f\u8457\u6dbc\u978b\u3001\u62d6\u978b\uff08\u8173\u8dbe\u4e0d\u8981\u88f8\u9732\uff09\uff0c\u914d\u6234\u773c\u93e1\u6216\u5b89\u5168\u8b77\u76ee\u93e1\u3002\u7559\u6709\u9577\u9aee\u8005\uff0c\u6234\u5e3d\u5957\u5c07\u982d\u9aee\u6372\u5165\u5957\u5167\uff0c\u6216\u4ee5\u6a61\u76ae\u5708\u675f\u65bc\u5f8c\uff0c\u4ee5\u9632\u6b62\u5f15\u706b\u5371\u96aa\u6216\u6c61\u67d3\u5be6\u9a57\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">3.\u5728\u5be6\u9a57\u5ba4\u5167\u7981\u6b62\u5438\u7159\u3001\u5403\u6771\u897f\u3001\u98f2\u98df\u3001\u5316\u599d\u3001\u56bc\u53e3\u9999\u7cd6\u3001\u5b09\u6232\u5954\u8dd1\uff0c\u98df\u7269\u98f2\u6599\u52ff\u5b58\u653e\u65bc\u5be6\u9a57\u5ba4\u7684\u51b0\u7bb1\u4e2d\uff0c\u5be6\u9a57\u684c\u4e0a\u52ff\u5806\u653e\u66f8\u5305\u3001\u66f8\u7c4d\u3001\u8863\u670d\u5916\u5957\u53ca\u96dc\u7269\u7b49\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">4.\u6240\u6709\u5be6\u9a57\u5100\u5668\u3001\u8017\u6750\u3001\u85e5\u54c1\u7b49\u5747\u5c6c\u5be6\u9a57\u5ba4\u6240\u6709\uff0c\u4e0d\u5f97\u651c\u51fa\u5be6\u9a57\u5ba4\u5916\u3002\u6bcf\u7d44\u5206\u914d\u4e4b\u5100\u5668\u3001\u8017\u6750\u8acb\u78ba\u5b9a\u6e05\u9ede\u8207\u4fdd\u7ba1\uff0c\u8ab2\u7a0b\u7d50\u675f\u5f8c\u5982\u6578\u6e05\u9ede\u7e73\u56de\u3002\u516c\u7528\u5100\u5668\u8acb\u5584\u52a0\u611b\u60dc\u4f7f\u7528\uff0c\u5be6\u9a57\u524d\u5f8c\uff0c\u8acb\u628a\u5de5\u4f5c\u5340\u57df\u6e05\u7406\u64e6\u62ed\uff0c\u4e26\u96a8\u6642\u4fdd\u6301\u74b0\u5883\u6e05\u6f54\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">5.\u5be6\u9a57\u524d\u8a73\u95b1\u5be6\u9a57\u5167\u5bb9\uff0c\u77ad\u89e3\u5be6\u9a57\u7d30\u7bc0\u7684\u539f\u7406\u53ca\u64cd\u4f5c\uff0c\u6ce8\u610f\u4e0a\u8ab2\u6240\u544a\u77e5\u7684\u6ce8\u610f\u4e8b\u9805\u3002\u5be6\u9a57\u9032\u884c\u4e2d\u6709\u4efb\u4f55\u72c0\u6cc1\u6216\u7591\u554f\uff0c\u96a8\u6642\u767c\u554f\uff0c\u5207\u52ff\u79c1\u81ea\u8b8a\u66f4\u5be6\u9a57\u7a0b\u5e8f\u3002\u6253\u7ffb\u4efb\u4f55\u85e5\u54c1\u8a66\u5291\u53ca\u5668\u76bf\u6642\uff0c\u8acb\u96a8\u5373\u6e05\u7406\u3002\u5be6\u9a57\u5f8c\uff0c\u9069\u5207\u8a18\u4e0b\u81ea\u5df1\u7684\u7d50\u679c\uff0c\u56b4\u7981\u6284\u8972\uff0c\u78ba\u5be6\u95dc\u9589\u4e0d\u7528\u4e4b\u96fb\u6e90\u3001\u6c34\u3001\u9152\u7cbe\u71c8\u53ca\u74e6\u65af\u7b49\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">6.\u7761\u7720\u4e0d\u8db3\u3001\u7cbe\u795e\u4e0d\u6fdf\u6216\u6ce8\u610f\u529b\u7121\u6cd5\u96c6\u4e2d\uff0c\u8acb\u7acb\u5373\u505c\u6b62\u5be6\u9a57\u3002\u5be6\u9a57\u6642\u9593\u82e5\u5ef6\u9577\uff0c\u8acb\u6ce8\u610f\u6642\u9593\u7684\u7ba1\u5236\u53ca\u81ea\u8eab\u7684\u5b89\u5168\uff0c\u4e0d\u53ef\u81ea\u884c\u9017\u7559\u5be6\u9a57\u5ba4\u904e\u591c\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">7.\u5be6\u9a57\u5b8c\u7562\uff0c\u8acb\u6e05\u7406\u5be6\u9a57\u5ba4\u3001\u5012\u5783\u573e\u3001\u6ec5\u83cc\u3001\u95dc\u9589\u71c8\u5149\u53ca\u51b7\u6c23\uff0c\u96e2\u958b\u5be6\u9a57\u5ba4\u524d\u8a18\u5f97\u6d17\u624b\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">8.\u4efb\u4f55\u610f\u5916\u4e8b\u4ef6\u61c9\u7acb\u5373\u5831\u544a\u5e2b\u9577\uff0c\u4e26\u61c9\u719f\u77e5\u76f8\u95dc\u4e4b\u61c9\u8b8a\u63aa\u65bd\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 14pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\"><strong>\u85e5\u54c1 <\/strong><br \/>\n\t\t<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">1.\u4f7f\u7528\u4efb\u4f55\u85e5\u54c1\uff0c\u8acb\u5148\u770b\u6e05\u695a\u6a19\u793a\u3001\u6ce8\u610f\u4e8b\u9805\uff0c\u7ffb\u95b1\u7269\u8cea\u5b89\u5168\u8cc7\u6599\u8868\u6216Merck Index\uff0c\u67e5\u660e\u662f\u5426\u5c0d\u4eba\u9ad4\u9020\u6210\u50b7\u5bb3\uff0c\u4f7f\u7528\u5b8c\u7562\u8acb\u653e\u56de\u539f\u4f4d\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">2.\u65b0\u914d\u88fd\u7684\u8a66\u5291\u8acb\u6e05\u695a\u8a3b\u660e\u5167\u5bb9\u7269\u3001\u6fc3\u5ea6\u3001\u6ce8\u610f\u4e8b\u9805\u53ca\u914d\u88fd\u65e5\u671f\uff0c\u70ba\u907f\u514d\u6c61\u67d3\uff0c\u52ff\u5c07\u672a\u7528\u5b8c\u7684\u85e5\u5291\u5012\u56de\u5bb9\u5668\u5167\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">3.\u63ee\u767c\u6027\u3001\u8150\u8755\u6027\u3001\u6709\u6bd2\u6eb6\u5291\uff08\u5982\u7532\u9187\u3001\u4e19\u916e\u3001\u918b\u9178\u3001\u6c2f\u4eff\u3001\u9e7d\u9178\u3001\u786b\u9178\u3001<\/span><span style=\"font-family:Symbol\">b<\/span><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">-mercaptoethanol\u3001formaldehyde\u3001\u915a\u7b49\uff09\u8981\u5728\u6392\u7159\u6ac3\u4e2d\u6234\u624b\u5957\u91cf\u53d6\u914d\u88fd\uff0c\u53d6\u7528\u5b8c\u61c9\u96a8\u5373\u84cb\u597d\u84cb\u5b50\uff0c\u82e5\u4e0d\u5c0f\u5fc3\u6253\u7ffb\u8a66\u5291\uff0c\u99ac\u4e0a\u8655\u7406\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">4.\u6709\u6bd2\u3001\u81f4\u764c\u85e5\u5291\u4f8b\u5982acrylamide\uff08\u795e\u7d93\u6bd2\uff09\u3001ethidium bromide\uff08\u7a81\u8b8a\u5291\uff09\u3001SDS\uff08\u7c89\u5875\uff09\u8acb\u6234\u624b\u5957\u53ca\u53e3\u7f69\u53d6\u7528\uff0c\u4e26\u52ff\u5230\u8655\u6c61\u67d3\uff0c\u812b\u4e0b\u624b\u5957\u5f8c\uff0c\u990a\u6210\u6d17\u624b\u7684\u597d\u7fd2\u6163\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">5.\u63a5\u89f8\u5230\u75c5\u539f\u6750\u6599\u6216\u7d30\u83cc\uff0c\u61c9\u8fc5\u901f\u6d88\u6bd2\u3002\u6240\u6709\u88ab\u6c61\u67d3\u7684\u7269\u54c1\uff0c\u5728\u4e1f\u68c4\u6216\u91cd\u8986\u4f7f\u7528\u524d\u5747\u9700\u5148\u6ec5\u83cc\uff0c\u6b32\u9ad8\u58d3\u6ec5\u83cc\u4e4b\u7269\u54c1\uff0c\u61c9\u653e\u5728\u5c08\u7528\u7684\u6536\u96c6\u6876\u5167\u3002\u56fa\u9ad4\u57f9\u990a\u57fa\u6216\u6d0b\u83dc\u81a0\u4e0d\u5f97\u5012\u5165\u6c34\u69fd\u6216\u4e0b\u6c34\u9053\u4e2d\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">6.\u4f7f\u7528\u523b\u5ea6\u5438\u7ba1\u53d6\u7269\u6642\uff0c\u5207\u52ff\u7528\u5634\u5438\u53d6\uff0c\u8acb\u7528\u81ea\u52d5\u5438\u7ba1\u5527\u7b52\u6216\u5b89\u5168\u5438\u7403\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 14pt\">\u00a0<br \/>\n\u00a0<\/p>\n<p style=\"text-align: justify;margin-left: 14pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\"><strong>\u5100\u5668 <\/strong><br \/>\n\t\t<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">1.\u4f7f\u7528\u5100\u5668\u524d\u5148\u77ad\u89e3\u5176\u6027\u80fd\u3001\u914d\u5099\u53ca\u6b63\u78ba\u64cd\u4f5c\u65b9\u6cd5\uff0c\u96f6\u4ef6\u53ca\u9644\u4ef6\u56b4\u7981\u62c6\u5378\uff0c\u52ff\u79c1\u81ea\u8abf\u6574\uff0c\u4e26\u6ce8\u610f\u63d2\u5ea7\u96fb\u58d3\uff08110V\u6216220V\uff09\u4e4b\u985e\u5225\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">2.\u4f7f\u7528\u96e2\u5fc3\u6a5f\u6642\uff0c\u96e2\u5fc3\u7ba1\u8981\u5169\u5169\u5c0d\u7a31\u3001\u91cd\u91cf\u5e73\u8861\uff0c\u9396\u7dca\u96e2\u5fc3\u8f49\u9640\u3002\u51b7\u51cd\u96e2\u5fc3\u6a5f\u65bc\u958b\u6a5f\u72c0\u614b\u6642\uff0c\u52d9\u5fc5\u84cb\u7dca\u84cb\u5b50\uff0c\u4ee5\u4fdd\u6301\u96e2\u5fc3\u69fd\u4e4b\u4f4e\u6eab\u4e26\u907f\u514d\u7d50\u971c\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">3.\u96fb\u6e90\u4f9b\u61c9\u5668\u6709\u9ad8\u96fb\u58d3\uff0c\u5207\u52ff\u89f8\u6478\u96fb\u6975\u6216\u96fb\u6cf3\u69fd\u5167\u6eb6\u6db2\uff0c\u624b\u6fd5\u5207\u52ff\u958b\u555f\u96fb\u6e90\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">4.\u4f7f\u7528\u5fae\u6ce2\u7210\u52a0\u71b1\uff0c\u4e0d\u53ef\u6709\u92c1\u7b94\u7b49\u91d1\u5c6c\u7269\u54c1\uff0c\u74f6\u84cb\u5fc5\u9808\u9b06\u958b\uff0c\u4ee5\u514d\u7206\u70b8\u3002\u52a0\u71b1\u5f8c\u6234\u9632\u71b1\u624b\u5957\u53d6\u51fa\u74f6\u5b50\uff0c\u52d9\u5fc5\u8f15\u6416\u6643\uff0c\u78ba\u8a8d\u4e0d\u6703\u7a81\u6cb8\u3002\u6b64\u5916\uff0c\u6ec5\u83cc\u65a7\u3001\u96fb\u78c1\u7210\u3001\u4e7e\u6d74\u5668\u7b49\uff0c\u4f7f\u7528\u524d\u719f\u6089\u64cd\u4f5c\u624b\u7e8c\uff0c\u56b4\u9632\u7206\u70b8\u6216\u71d9\u50b7\u3002<br \/>\n<\/span><\/p>\n<p style=\"text-align: justify;margin-left: 27pt\"><span style=\"font-family:\u65b0\u7d30\u660e\u9ad4\">5.\u89c0\u6e2cUV\u71c8\u6642\uff0c\u4e0d\u8981\u4ee5\u773c\u775b\u76f4\u8996UV\uff0c\u61c9\u9694\u8457\u6a94\u677f\u89c0\u5bdf\uff0c\u5b8c\u7562\u7acb\u5373\u95dc\u71c8\uff0c\u52d9\u5fc5\u6234\u624b\u5957\u64cd\u4f5c\u4ee5\u9632ethidium bromide\u6c61\u67d3\u3002<br 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